Advances in lignocellulosic biotechnology: A brief review on lignocellulosic biomass and cellulases

Extrusion Pretreatment of Lignocellulosic Biomass: A Review

Bioconversion of lignocellulosic biomass to bioethanol has shown environmental, economic and energetic advantages in comparison to bioethanol produced from sugar or starch. However, the pretreatment process for increasing the enzymatic accessibility and improving the digestibility of cellulose is hindered by many physical-chemical, structural and compositional factors, which make these material...

Chemical and Physicochemical Pretreatment of Lignocellulosic Biomass: A Review

Overcoming the recalcitrance (resistance of plant cell walls to deconstruction) of lignocellulosic biomass is a key step in the production of fuels and chemicals. The recalcitrance is due to the highly crystalline structure of cellulose which is embedded in a matrix of polymers-lignin and hemicellulose. The main goal of pretreatment is to overcome this recalcitrance, to separate the cellulose f...

Microbial Degradation of Lignocellulosic Biomass

Recycling of cellulases in lignocellulosic hydrolysates using alkaline elution.

The recovery of cellulases from lignin, lignocellulosic hydrolysates and cellulose by alkaline washes at pH 9 and 10 was examined. The effect of the pH on the structural stability of purified Cel7A was analyzed by circular dichroism. Purified Cel7A showed conformational changes at pH 9 and 10 that were reversible at pH 4.8. Temperature influenced the enzymatic hydrolysis of wheat straw and may ...

Hydrothermal Pretreatment of Lignocellulosic Biomass and Kinetics

The study focus was an examination of the hydrothermal pretreatment method applied to the lignocellulosic substrate, represented by the prairie cord grass, and comparison between different conditions based on the yield of glucose after enzymatic hydrolysis. The treatment did not involve any chemicals usage. Enzymatic hydrolysis was performed in order to examine the amount of glucose which was r...